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You are here:Home » Molecular Biology » MB-BioSep Blocking Reagents » Skim Milk Agar (Plate Count)

Skim Milk Agar (Plate Count)

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Specifications

For determining the microbial count in milk and dairy products. The culture medium complies with the recommendations of the International Dairy Federation (Internationaler Milchwirtschafts-verband) (1991) and the DIN Norm 10192 for the examination of milk and dairy products.
Catalog #S1013-90M
Addition of skim milk to a culture medium with a superior nutrient base optimally adapts it to the neutral conditions experienced by microorganisms which grow in milk. More colonies grow and a wider range of bacteria can be cultured than in other culture media intended for the same purpose (TERPLAN et al. 1967).
AppearanceOff-white to tan, homogeneous, free flowing powder
Solubility:
Colorless to yellow, clear to opalescent, complete after autoclaving.
pH:
As Reported
Formulation (g/Liter)
Agar 10.5
Glucose 1.0
Skim Milk powder (no inhibitors) 1.0
Casein Peptone 5.0
Yeast Extract 2.5
Total20g/Liter
Directions per Liter
Suspend 20.0 g/liter in cold water and allow to stand for about 15 minutes. Transfer flask to a cold water bath and heat gently, with frequent shaking, until completely dissolved, then autoclave (15 min at 121°C).
Storage and Stability
Store powdered media at 4ºC. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.


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