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Skim Milk Agar (Plate Count)
Skim Milk Agar (Plate Count)
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| For determining the microbial count in milk and dairy products. The culture medium complies with the recommendations of the International Dairy Federation (Internationaler Milchwirtschafts-verband) (1991) and the DIN Norm 10192 for the examination of milk and dairy products. | | | Catalog # | S1013-90M | | Addition of skim milk to a culture medium with a superior nutrient base optimally adapts it to the neutral conditions experienced by microorganisms which grow in milk. More colonies grow and a wider range of bacteria can be cultured than in other culture media intended for the same purpose (TERPLAN et al. 1967). | | Appearance | Off-white to tan, homogeneous, free flowing powder | | Solubility: | | Colorless to yellow, clear to opalescent, complete after autoclaving. | | pH: | | As Reported | | Formulation (g/Liter) | | | Agar 10.5 | | Glucose 1.0 | | Skim Milk powder (no inhibitors) 1.0 | | Casein Peptone 5.0 | | Yeast Extract 2.5 | | | Total | 20g/Liter | | | Directions per Liter | | | Suspend 20.0 g/liter in cold water and allow to stand for about 15 minutes. Transfer flask to a cold water bath and heat gently, with frequent shaking, until completely dissolved, then autoclave (15 min at 121°C). | | | Storage and Stability | | | Store powdered media at 4ºC. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time. |
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