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You are here:Home » Antibodies » Abs to Adapter Proteins » Anti -SLP 76 (SH2-domain Leukocyte Protein)

Anti -SLP 76 (SH2-domain Leukocyte Protein)


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Clone Host Grade Applications
Polyclonal Sheep Affinity Purified B IP
SLP 76 was recently identified as a substrate of the T cell receptor stimulated protein tyrosine kinase. This molecule is rapidly phosphorylated on T cell receptor activation. SLP 76 has three identifiable domains; the first containing a number of tyrosines which are the likely sites of phosphorylation, the second a proline-rich domain, and the third an SH2 domain. SLP 76 has been shown to bind to the Grb2 adapter protein in vivo; the association between Grb2 and SLP 76 has been mapped to a 20 amino acid stretch in SLP76's proline-rich region. The SH2 domain of SLP 76 binds to two tyrosine phosphorylated proteins after T cell receptor activation. These molecules are yet to be identified. The SH2 domain of SLP 76 also appears to bind to a serine threonine kinase, perhaps via an indirect association.. Interestingly, overexpression of SLP 76 results in greatly augmented IL-2 production in transiently transfected T cells. Collectively, these data suggest that SLP 76 plays an important role in T cell activation and perhaps is involved in coupling signaling pathways after activation of the T cell antigen receptor. Immunogen: Bacterially expressed GST-SLP 76 fusion protein translated from the cloned human cDNA.
Catalog #S1014-20
ApplicationsSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.
Recommended DilutionWestern Blot: 0.5-2ug/ml.. Detects SLP 76 in RIPA lysates from Jurkat cells.
Immunoprecipitation: 4ug from a previous lot immunoprecipitated SLP 76 from 500ug of Jurkat cell RIPA lysate. Optimal dilutions to be determined by the researcher.
Included Positive Antigen ControlJ9000: Jurkat lysate. Add 2.5ul of 2-mercaptoethanol/100ul of lysate and boil for 5 minutes to reduce the preparation. Load 20ug of reduced lysate per lane for minigels.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
FormSupplied as a solution in 250ul of 0.1M Tris-glycine, pH 7.5, 150mM sodium chloride, 0.05% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenBacterially expressed GST-SLP 76 fusion protein.
SpecificityRecognizes human SLP 76 at ~76kD.
Species CrossreactivityMouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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