You are here:Home
»
Antibodies
»
Abs to T Cells
»
Anti -SLP 76, phosphorylated (Tyr145) (SH2-domain Leukocyte Protein)
Anti -SLP 76, phosphorylated (Tyr145) (SH2-domain Leukocyte Protein)
Pricing
For pricing information, USA customers sign in. Outside USA? Please contact your distributor for pricing. Specifications
| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Rabbit |
Supernatant |
B IC FC |
|
| The activation of protein tyrosine kinases is a critical event in T cell antigen receptor (TCR)-mediated signaling. One substrate of the TCR-activated protein tyrosine kinase pathway is a 76-kD protein (SLP-76) that associates with the adaptor protein Grb2. Northern analysis demonstrates that SLP76 mRNA is expressed solely in peripheral blood leukocytes, thymus, and spleen; and in human T cell, B cell and monocytic cell lines. In vitro translation of SLP76 cDNA gives rise to a single product of 76kD that associates with a GST/Grb2 fusion protein, demonstrating a direct association between these two molecules. It has been reported that this novel protein is likely to play an important role in TCR-mediated intracellular signal transduction (1). It has been shown that SDF-1alpha stimulation of prolonged ERK activation in Jurkat T cells requires both the ZAP-70 tyrosine kinase and the Src homology 2 domain-containing leukocyte protein of SLP-76 scaffold protein. This pathway involves ZAP-70-dependent tyrosine phosphorylation of SLP-76 at one or more of its tyrosines, 113, 128, and 145 (2). | | | Catalog # | S1014-20L | | Applications | Suitable for use inFlow Cytometry, Western Blot, Immunocytochemistry. Other applications not tested. | | Recommended Dilution | Western Blot: 1:100,000-1:200,000 | | Immunocytochemistry: 1:100 | | Flow Cytometry: 1:100 | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG | | Clone No | 9E550 | | Host | Rabbit | | Source | Human | | Concentration | Not determined | | Form | Supplied as a liquid in 50 mM Tris-Glycine, pH 7.4, 0.15 M sodium chloride, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. | | Purity | Supernatant | | Immunogen | A phospho specific peptide corresponding to residues surrounding tyrosine 145 of human SLP-76. | | Specificity | Recognizes human SLP-76 phosphorylated at Tyr145. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. | | Alternate names | SH2 domain-containing leukocyte protein of 76kD, SLP-76 tyrosine phosphoprotein, SLP76 |
External Links
|
