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You are here:Home » Antibodies » Abs to Autoimmune » Anti -Sm (Smith) Antigen

Anti -Sm (Smith) Antigen

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Specifications

Clone Host Grade Applications
Polyclonal Human Purified E IP Gel
The sm (Smith) is an autoantigen on a series of particles composed of RNA and protein. Unlike RNP, the sm antigen is resistant to RNase, but partially sensitive to trypsin. It is involved in the pathogenesis of Systemic lupus erythematosus (SLE). Antibodies to sm are found in 20-30% of patients with SLE.
Catalog #S1014-25
ApplicationsSuitable for use in ELISA, Immunodiffusion and Immunoprecipitation. Other applications not tested.
Recommended DilutionELISA: 1:100
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol, aliquot and store at -20°C. Reconstituted product is stable for12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostHuman
ConcentrationNot determined.
FormSupplied as a lyophilized powder in 0.01 M PBS, 0.02% sodium azide. Reconstitute with 2ml sterile, ddH2O or sterile 40-50% glycerol.
PurityPurified by delipidation, defibrination and ammonium sulfate precipitation.
ImmunogenDerived from donor human plasma.
SpecificityRecognizes human Sm. Specificity is verified by a precipitin line of identity with the CDC-AF reference serum. Crossreactivity: RNP
and anti-SSA (low levels). No reactivity at a dilution of 1100 when tested against purified SSB, Scl-70 and Jo-1 in an ELISA system designed to detect all IgG subclasses.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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