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Specifications

Clone Host Grade Applications Polyclonal Human Purified E IP Gel

The sm (Smith) is an autoantigen on a series of particles composed of RNA and protein. Unlike RNP, the sm antigen is resistant to RNase, but partially sensitive to trypsin. It is involved in the pathogenesis of Systemic lupus erythematosus (SLE). Antibodies to sm are found in 20-30% of patients with SLE.
Catalog #	S1014-25
Applications	Suitable for use in ELISA, Immunodiffusion and Immunoprecipitation. Other applications not tested.
Recommended Dilution	ELISA: 1:100
	Optimal dilutions to be determined by the researcher.
Storage and Stability	Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol, aliquot and store at -20°C. Reconstituted product is stable for12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Human
Concentration	Not determined.
Form	Supplied as a lyophilized powder in 0.01 M PBS, 0.02% sodium azide. Reconstitute with 2ml sterile, ddH2O or sterile 40-50% glycerol.
Purity	Purified by delipidation, defibrination and ammonium sulfate precipitation.
Immunogen	Derived from donor human plasma.
Specificity	Recognizes human Sm. Specificity is verified by a precipitin line of identity with the CDC-AF reference serum. Crossreactivity: RNP
and anti-SSA (low levels). No reactivity at a dilution of 1	100 when tested against purified SSB, Scl-70 and Jo-1 in an ELISA system designed to detect all IgG subclasses.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.