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You are here:Home » Antibodies » Antibodies-Autoimmune » Anti -Sm (Smith) Antigen

Anti -Sm (Smith) Antigen


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Clone Host Grade Applications
Polyclonal Human Purified E
The sm (Smith) is an autoantigen on a series of particles composed of RNA and protein. Unlike RNP, the sm antigen is resistant to RNase, but partially sensitive to trypsin. It is involved in the pathogenesis of Systemic lupus erythematosus (SLE). Antibodies to sm are found in 20-30% of patients with SLE.
Catalog #S1014-25A
ApplicationsSuitable for use in ELISA and Immunodiffusion. Other applications not tested.
Recommended DilutionELISA: 1:100
Optimal dilutions to be determined by the researcher.
TiterImmunodiffusion: 1:2
Other Autoantibody Activity in the SerumThe serum exhibits no reactivity at a dilution of 1:100 when tested against purified SSA, SSB, SCL, Jo-1, mitochondrial or ribosomal antigens in an ELISA system designed to detect IgG antibody subclasses.
Interpretation of Results: <16EU/ml: Negative; 16-20EU/ml: Equivocal; Positive; >20EU/ml:
SSA: 3EU/ml
SSB: 3EU/ml
SCL: 4EU/ml
Sm: 695EU/ml
Sm/RNP: 220EU/ml
Jo-1: 5EU/ml
Interpretation of ResultsNegative: <16EU/ml, Equivocal: 16-20EU/ml, Positive: >20EU/ml
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. 
Clone TypePolyclonal
ConcentrationNot Determined
FormSupplied as a lyophilized powder from PBS, pH 7.2, 0.02% sodium azide. Reconstitute with 2ml sterile ddH2O.
PurityPurified by fractionation of delipidated and defibrinated donor human serum.
ImmunogenSm Antigen. Autoantibody derived from human serum.
SpecificityRecognizes human Sm antigen. Specificity is verified by a precipitin line of identity with the CDC-AF reference serum. Anti-RNP precipitant is detected. This product shows no reactivity at a dilution of 1
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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