| The sm (Smith) is an autoantigen on a series of particles composed of RNA and protein. Unlike RNP, the sm antigen is resistant to RNase, but partially sensitive to trypsin. It is involved in the pathogenesis of Systemic lupus erythematosus (SLE). Antibodies to sm are found in 20-30% of patients with SLE. |
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| Catalog # | S1014-29BX |
| Applications | Suitable for use in Electron Microscopy, Immunofluorescence, Western Blot, Immunohistochemistry and Affinity Purification. Other applications have not been tested. |
| Recommended Dilutions | Western Blot: 0.5-1.0ug/ml for 2 hours at RT |
| Immunohistochemistry (Formalin/paraffin): 1:50-1:100 for 30 minute at RT. No special pretreatment is required for immunohistochemical staining of formalin/paraffin tissues. |
| Optimal dilutions to be determined by the researcher. |
| Positive Control | LS174T cells, Tonsil and Testis |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG3,k |
| Clone No | 3F332 |
| Host | Mouse |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.4. Also available with BSA and azide. See S1014-29B. |
| Purity | Purified by Protein G affinity chromatography. |
| Immunogen | Sm (Smith) Antigen. Cellular Localization: Nuclear. |
| MW of Antigen | ~25kD (predominant) |
| Specificity | Recognizes crossreactive epitopes on the B’/B and D polypeptides of Sm. Precipitates the small nuclear RNAs U1, U2, U4, U5, and U6 providing direct evidence that the Sm antigen resides on all these RNA-protein complexes. Species crossreactivity: human, porcine, rabbit, rat and Drosophila. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
