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You are here:Home » Antibodies » Abs to Smad Proteins » Anti -Smad1, Smad5, Smad8, phosphorylated (Ser463,465), (Ser463,465), (Ser426,428) (Small Mothers Against Decapentaplegic Deleted in Pancreatic Carcinoma)

Anti -Smad1, Smad5, Smad8, phosphorylated (Ser463,465), (Ser463,465), (Ser426,428) (Small
Mothers Against Decapentaplegic Deleted in Pancreatic Carcinoma)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IP
Bone morphogenetic proteins (BMPs) constitute a large family of signaling molecules that regulate a wide range of critical processes including morphogenesis, cell-fate determination, proliferation, differentiation and apoptosis. BMP receptors are members of the TGF-beta family of Ser/Thr kinase receptors. Ligand binding induces multimerization, autophosphorylation and activation of these receptors. They subsequently phosphorylate Smad1 at Ser463 and Ser465 in the carboxy-terminal motif SSXS, as well as Smad5 and Smad8 at their corresponding sites. These phosphorylated Smads dimerize with the coactivating Smad4 and translocate to the nucleus, where they stimulate transcription of target genes.
Catalog #S1014-51D
ApplicationsSuitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested.
Recommended DilutionsWestern Blot: 1:1000
Immunoprecipitation: 1:100
Chromatin Immunoprecipitation: 1:50
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot Determined
FormSupplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
PurityPurified by Protein A and peptide affinity chromatography.
ImmunogenSynthetic phosphopeptide corresponding to residues around Ser463/465 of human Smad5.
SpecificityRecognizes endogenous levels of human Smad1 at ~60kD only when dually phosphorylated at Ser463 and Ser465 as well as human Smad5 and Smad8 only when phosphorylated at the equivalent sites. Does not crossreact with other Smad-related proteins. Species Crossreactivity: mouse, rat, mink and Xenopus
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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