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You are here:Home » Antibodies » Abs to Smad Proteins » Anti -Smad1, phosphorylated (Ser465) (Small Mothers Against Decapentaplegic Deleted in Pancreatic Carcinoma)

Anti -Smad1, phosphorylated (Ser465) (Small Mothers Against Decapentaplegic Deleted in
Pancreatic Carcinoma)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
Smad proteins, the mammalian homologs of the Drosophila Mothers against dpp (Mad), are downstream effectors of TGFb/BMP signaling. Smad1 (MADR1) mediates signals of bone morphogenetic proteins (BMPs) in the cell. BMPs constitute a large family of signaling molecules that regulate a wide range of critical processes including morphogenesis, cell-fate determination, proliferation, differentiation and apoptosis. BMP receptors are members of the TGFb family of
Ser/Thr kinase receptors. Ligand binding induces multimerization, autophosphorylation and activation of these receptors. Subsequently, they phosphorylate Smad1 at serine 463 and 465 in the carboxyl-terminal motif SSXS, as well as Smad5 and Smad8 at their corresponding sites. These phosphorylated Smads dimerize with the collaborating Smad4 (DPC4) and are translocated into the nucleus, where the transcription of target genes is stimulated.
Catalog #S1014-51H
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1:500-1:1000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS (Mg2+ and Ca2+ free), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthesized phosphopeptide derived from human Smad1 around the phosphorylation site of serine 465 (I-S-S-V-SP).
SpecificityRecognizes endogenous levels of human Smad1 only when phosphorylated at serine 465.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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