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You are here:Home » Antibodies » Abs to Smad Proteins » Anti -Smad1, Smad5, Smad8, Smad9 (SMAD-1, SMAD-5, SMAD-8, SMAD8A, SMAD8B, SMAD-9, BSP1, BSP-1, CMT2D, DPC4, GARS, HMN5, hSMAD1, hSMAD4, JV41, JV51, MAD Homolog 5, Mad-related Protein 1, MADR1, Madh6, Mothers Against Decapentaplegic Homolog 1, Mothers Against DP

Anti -Smad1, Smad5, Smad8, Smad9 (SMAD-1, SMAD-5, SMAD-8, SMAD8A, SMAD8B, SMAD-9, BSP1,
BSP-1, CMT2D, DPC4, GARS, HMN5, hSMAD1, hSMAD4, JV41, JV51, MAD Homolog 5, Mad-related
Protein 1, MADR1, Madh6, Mothers Against Decapentaplegic Homolog 1, Mothers Against DP

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
SMADs are members of the MAD-related family of molecules. MAD-related proteins are a family of intracellular proteins that are essential components in the signaling pathways of the serine/threonine kinase receptors of the transforming growth factor beta superfamily (1). SMADs can be divided into receptor-regulated SMADs (R-SMADs: SMAD1, SMAD2, SMAD5, SMAD8 and SMAD9), common-mediator SMAD (co-SMAD: SMAD4) and inhibitory SMADs (I-SMADs: SMAD6 and SMAD7). SMAD1, SMAD5, SMAD8 and SMAD9 have high degrees of homology and antibodies are available that recognize sequences common to all of them. SMAD8 and SMAD9 are typically used as alternate names for one another in the literature. Human SMAD1 is a 465aa protein. Human SMAD2 is a 467aa protein. Human SMAD3 is a 425aa protein. Human SMAD4 is a 552aa protein. Human SMAD5 is a 465aa protein. Human SMAD6 is a 496aa protein. Human SMAD7 is a 426aa protein. Mouse SMAD8 is a 430aa protein. Human SMAD9 is a 430aa protein.
Catalog #S1014-51T
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1-3ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenTwo synthetic peptides corresponding to aa18-32 and aa315-330 ((LGWKQGDEEEKWAEK); (SNVNRNSTIENTRRHI) from human SMAD1 (GenBank Accession #AAP36050.1). Sequence Homology (100%): SMAD5, SMAD8, SMAD9.
SpecificityRecognizes human Smad1, Smad5, Smad8, Smad9. May crossreact with SMAD2 or SMAD3. The actual SMADs detected may vary according to cell or tissue-type specific expression. Species Crossreactivity: Mouse, rat, chicken.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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