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You are here:Home » Antibodies » Abs to Smad Proteins » Anti -Smad2 (MADH2, Small Mothers Against Decapentaplegic Deleted in Pancreatic Carcinoma)

Anti -Smad2 (MADH2, Small Mothers Against Decapentaplegic Deleted in Pancreatic Carcinoma)

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Supernatant B IP IH IC FC
Smad 2 is a member of the Mothers Against Dpp (MAD)-related family of proteins. So far, nine Smads have been identified and can be divided in 3 subgroups based on their structure and functions; pathway-restricted, common mediator and inhibitory Smad. Smad 2 and 3 serve as pathway-restricted Smads for the TGF-beta/activin signaling pathways (1-2). Smad 2 is phosphorylated by the TGF-beta type 1 receptor kinase on Ser465 and Ser467. Phosphorylation of both serines is essential for Smad 2-Smad 4 heteromeric complex and dissociation of Smad 2 from the TGF-beta type 1 receptor (3). Once phosphorylated Smad 2 translocate to the nucleus where it associates with DNA-binding proteins and forming a transcriptional complex (4). Unlike Smad 3 and 4, Smad 2 does not directly bind DNA (5).
Catalog #S1014-52C
ApplicationsSuitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended DilutionFlow Cytometry: 1:30
Western Blot: 1:10,000
Immunoprecipitation: 1:50
Immunohistochemistry: 1:100-1:250
Immunocytochemistry: 1:100-1:250
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
Clone TypeMonoclonal
IsotypeIgG
Clone No6k373
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in 50mM Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% BSA, 0.01% sodium azide and 40% glycerol.
PuritySupernatant
ImmunogenA synthetic peptide corresponding to residues (between amino acids 75 and 105) surrounding the MH2 domain of human Smad2.
SpecificityRecognizes human Smad2 at ~58kD. Species Crossreactivity: Rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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