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You are here:Home » Antibodies » Abs to Smad Proteins » Anti -Smad2 (SMAD-2, hMAD-2, hSMAD2, JV18-1, JV18, MAD-related Protein 2, MADR2, MGC22139, MGC34440, Mothers Against Decapentaplegic Homolog 2, Mothers Against DPP Homolog 2, MADH2)

Anti -Smad2 (SMAD-2, hMAD-2, hSMAD2, JV18-1, JV18, MAD-related Protein 2, MADR2, MGC22139,
MGC34440, Mothers Against Decapentaplegic Homolog 2, Mothers Against DPP Homolog 2, MADH2)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Supernatant B IH IC FC
SMAD2 or Mothers against decapentaplegic homolog 2 is a polypeptide that, as its name describes, is a homolog of the Drosophila gene: "Mothers against decepentaplegic". It belongs to the SMAD family of proteins, which belong to the TGFbeta superfamily of modulators. Like many other TGF beta family members SMAD2 is involved in cell signalling. SMAD2 modulates signals of activin and TGFbeta's. It interacts with SMAD anchor for receptor activation (SARA). The binding of ligands causes the phosphorylation of the SMAD2 protein and the dissociation from SARA and the association with SMAD4. It is subsequently transferred to the nucleus where it forms complexes with other proteins and acts as a transcription factor. SMAD2 is a receptor regulated SMAD (R-SMAD) and is activated by bone morphogenetic protein type 1 receptor kinase.
Catalog #S1014-52G
ApplicationsSuitable for use in Flow Cytometry, Western Blot, Immunohistochemistry, and Immunocytochemistry. Other applications not tested.
Recommended DilutionFlow Cytometry: 1:20
Western Blot: 1:500-1000
Immunohistochemistry (paraffin): 1:50
Immunocytochemistry: 1:250-500
Optimal dilutions to be determined by the researcher.
Positive ControlJurkat cell lysate
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG
Clone No9i209
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.01% sodium azide, 0.05% BSA, 40% glycerol.
PuritySupernatant
ImmunogenSynthetic peptide corresponding to residues surrounding the MH2 domain of human SMAD2. Epitope: MH2 domain
SpecificityRecognizes human SMAD2 at and around the MH2 domain.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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