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You are here:Home » Antibodies » Abs to Smad Proteins » Anti -Smad2 (SMAD Family Member 2, SMAD 2, SMAD-2, hMAD-2, hSMAD2, JV18-1, JV18, MAD-related Protein 2, MADR2, Mothers Against Decapentaplegic Homolog 2, Mothers Against DPP Homolog 2, MAD Homolog 2, MADH2)

Anti -Smad2 (SMAD Family Member 2, SMAD 2, SMAD-2, hMAD-2, hSMAD2, JV18-1, JV18,
MAD-related Protein 2, MADR2, Mothers Against Decapentaplegic Homolog 2, Mothers Against
DPP Homolog 2, MAD Homolog 2, MADH2)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Supernatant B IP FC IF
Members of the Smad family of signal transduction molecules are components of a critical intracellular pathway that transmit TGF-B signals from the cell surface into the nucleus. Three distinct classes of Smads have been defined: the receptor-regulated Smads (R-Smads), which include Smad1, 2, 3, 5 and 8; the common-mediator Smad (co-Smad), Smad4; and the antagonistic or inhibitory Smads (I-Smads), Smad6 and 7 (1-5). Activated type I receptors associate with specific R-Smads and phosphorylate them on a conserved carboxy-terminal SSXS motif. The phosphorylated R-Smad dissociates from the receptor and forms a heteromeric complex with the co-Smad (Smad4), allowing translocation of the complex to the nucleus. Once in the nucleus, Smads can target a variety of DNA binding proteins to regulate transcriptional responses (6-8).
Catalog #S1014-52L
ApplicationsSuitable for use in Immunofluorescence, Flow Cytometry, Western Blot and Immunoprecipitation. Other applications not tested.
Recommended DilutionImmunofluorescence (IF-IC): 1:50
Flow Cytometry: 1:200
Western Blot: 1:1000 Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight.
Immunoprecipitation: 1:50
Chromatin Immunoprecipitation: 1:50
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG
Clone No11C1070
HostRabbit
SourceMouse
ConcentrationNot determined
FormSupplied a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, < 0.02% sodium azide, 50% glycerol.
PuritySupernatant
ImmunogenSynthetic peptide corresponding to residues near the amino terminus of mouse Smad2.
SpecificityRecognizes endogenous levels of total Smad2. Species Crossreactivity: human, rat, monkey.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesMGC22139, MGC34440


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