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You are here:Home » Antibodies » Abs to Smad Proteins » Anti -SMAD2, phosphorylated (Ser118) (Mothers Against Decapentaplegic Homolog 2, Mothers Against DPP Homolog 2, MAD Homolog 2, Mad-related Protein 2, hMAD-2, SMAD Family Member 2, SMAD 2, hSMAD2, JV18-1, MADR2, MADH2) (AP)

Anti -SMAD2, phosphorylated (Ser118) (Mothers Against Decapentaplegic Homolog 2, Mothers
Against DPP Homolog 2, MAD Homolog 2, Mad-related Protein 2, hMAD-2, SMAD Family Member 2,
SMAD 2, hSMAD2, JV18-1, MADR2, MADH2) (AP)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E Gel
The protein belongs to the SMAD, a family of proteins similar to the proteins of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin.
Catalog #S1014-52M-AP
ApplicationsSuitable for use in ELISA and Dot Blot. Other applications not tested.
Recommended DilutionELISA: 1:1,000
Dot Blot: 1:500
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Do not freeze alkaline phosphatase conjugates which could result in a substantial loss of enzymatic activity. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in PBS, pH 7.2. Contains ~0.09% sodium azide. Conjugated to Alkaline Phosphatase.
PurityPurified by Protein A affinity chromatography.
ImmunogenSynthetic phosphopeptide corresponding to amino acid residues surrounding Ser118 of human SMAD2 (KLH).
SpecificityRecognizes human SMAD2 when phosphorylated at Ser118.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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