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You are here:Home » Antibodies » Abs to Smad Proteins » Anti -Smad2,3 (Small Mothers Against Decapentaplegic Deleted in Pancreatic Carcinoma)

Anti -Smad2,3 (Small Mothers Against Decapentaplegic Deleted in Pancreatic Carcinoma)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
Catalog #S1014-53A
ApplicationsSuitable for use in Western Blot. Other applications have not been tested.
Recommended DilutionWestern Blot: 1:1000-1:2000 detects Smad2/3 in RIPA lysates of HepG2 cells. An additional unknown band was detected at Mr 97kD in the lysate.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide before the addition of glycerol to 30%.
PurityPurified by immunoaffinity chromatography.
ImmunogenFusion protein corresponding to amino acids 186-273 of human Smad2.
SpecificityRecognizes human Smad2 Mr 55-60kD and Smad3 Mr 50kD as determined by using Smad2 and Smad3 transfected Cos cells. Species Crossreactivity: Predicted to crossreact with mouse and rat based on conservation of immunogen sequence.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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