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Anti -Smad2,3 (Small Mothers Against Decapentaplegic Deleted in Pancreatic Carcinoma)
Anti -Smad2,3 (Small Mothers Against Decapentaplegic Deleted in Pancreatic Carcinoma)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Goat |
Affinity Purified |
B |
|
| Smads are a family of intracellular proteins that transmit transforming growth factor beta (TGF- ) superfamily signals from the cell surface to the nucleus. The Smad family is divided into three subclasses: receptor regulated Smads, (Smads 1, 2, 3, 5 and 8); the common partner, (Smad4); and the inhibitory Smads, (Smads 6 and 7). The binding of TGF- or activin to their cognate receptor induces phosphorylation of Smads 2 and 3. The activated Smads associate with the common-mediator subunit, Smad4, and the heteromeric complex translocates into the nucleus to initiate transcription. Smad3, also known as Mothers Against Decapentaplegic homolog 3 (MADH3), shares 83% amino acid identity with Smad2, also known as Mothers Against Decapentaplegic homolog 2 (MADH2). Human Smad2 has 99% identity to mouse and rat Smad2. Human Smad3 has 99% identity to mouse and rat Smad3. | | | | | | Reconstitution: | | | Reconstitute the antibody in 100 L PBS containing 0.02% NaN3. The antibody concentration will be 1.0 mg/mL. | | | | | | Applications: | | | Western Blot: 0.5ug/ml | | | Optimal dilutions should be determined by each laboratory for each application. | | | | | | Storage and Stability: | | | Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile PBS with 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | | Catalog # | S1014-53A1 | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Goat | | Source | Human | | Concentration | As reported | | Form | Supplied as a lyophilized powder from a 0.2um filtered solution in PBS with 5% trehalose. Reconstitute with sterile PBS, 40-50% glycerol. If 0.1ml is used, the concentration will be 1mg/ml. | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | E. coli derived recombinant human Smad3 (aa2-230) | | Specificity | Detects endogenous human and mouse Smad3 on Western Blots. Reacts with recombinant human Smad2 (Accession # Q15796) and Smad3 in Western Blots. Predicted to detect rat | | based on sequence homology. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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