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You are here:Home » Antibodies » Abs to Smad Proteins » Anti -SMAD2, 3 (Mothers Against Decapentaplegic Homolog 2, MAD Homolog 2, Mothers Against DPP Homolog 2, JV18-1, Mad-related Protein 2, hMAD-2, SMAD Family Member 2, SMAD 2, MADH2, MADR2/Mothers Against Decapentaplegic Homolog 3, MAD Homolog 3, Mad3, Mothers Ag

Anti -SMAD2, 3 (Mothers Against Decapentaplegic Homolog 2, MAD Homolog 2, Mothers Against
DPP Homolog 2, JV18-1, Mad-related Protein 2, hMAD-2, SMAD Family Member 2, SMAD 2, MADH2,
MADR2/Mothers Against Decapentaplegic Homolog 3, MAD Homolog 3, Mad3, Mothers Ag

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP FC IF
Members of the Smad family of signal transduction molecules are components of a critical intracellular pathway that transmit TGF-b signals from the cell surface into the nucleus. Three distinct classes of Smads have been defined: the receptor-regulated Smads (R-Smads), which include Smad1, 2, 3, 5, and 8; the common-mediator Smad (co-Smad), Smad4; and the antagonistic or inhibitory Smads (I-Smads), Smad6 and 7 (1-5). Activated type I receptors associate with specific R-Smads and phosphorylate them on a conserved carboxy-terminal SSXS motif. The phosphorylated R-Smad dissociates from the receptor and forms a heteromeric complex with the co-Smad (Smad4), allowing translocation of the complex to the nucleus. Once in the nucleus, Smads can target a variety of DNA binding proteins to regulate transcriptional responses (6-8).
Catalog #S1014-53A3
ApplicationsSuitable for use in Immunofluorescence, Flow Cytometry, Western Blot, Immunoprecipitation, Chromatin IP, Chip Aragose and Chip Magnetic. Other applications not tested.
Recommended DilutionImmunofluorescence (IF-IC): 1:125
Flow Cytometry: 1:200
Western Blot: 1:1000
Immunoprecipitation: 1:50
Chromatin IP: 1:50
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol.
PurityPurified by Protein A affinity chromatography.
ImmunogenSynthetic peptide corresponding to residues surrounding Ala208 of human Smad2.
SpecificityRecognizes endogenous levels of total human Smad2/3. Species Crossreactivity: Monkey, mouse, Xenopus and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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