Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Antibodies » Abs to Smad Proteins » Anti -Smad3 (Small Mothers Against Decapentaplegic Deleted in Pancreatic Carcinoma)

Anti -Smad3 (Small Mothers Against Decapentaplegic Deleted in Pancreatic Carcinoma)

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
Catalog #S1014-53G
ApplicationsSuitable for use in Western Blot. Other applications have not been tested.
Recommended DilutionWestern Blot: 2-4ug/ml detects Smad3 in RIPA lysates from HepG2 cells. HepG2 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Smad3 (2ug/ml) preincubated with or without immunogenic peptide. Proteins were visualized using a goat antirabbit secondary antibody conjugated to HRP and an enhanced chemiluminescence detection system.
Optimal dilutions to be determined by the researcher.
*Recommended secondary antibodiesI1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
I1904-06H IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
I1904-14C: IgG, Fc (HRP) Pab Gt xMo
I1904-19C: IgG, F(abí)2 (HRP) Pab Gt xMo
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, 30% glycerol.
PurityPurified by Protein A affinity chromatography.
ImmunogenSynthetic peptide corresponding to aa206-220
[CNLSPNPMSPAHNNLD-amide] of human Smad3 with a C-terminal cysteine for conjugation as shown by comparison with the immunizing peptide. (KLH conjugated)
SpecificityRecognizes Smad3, Mr ~50kD. Nonspecific bands were detected, Mr 55 & 66kD. Species Crossreactivity: Human, mouse and rat
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links