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You are here:Home » Antibodies » Abs to Smad Proteins » Anti -Smad3 (Small Mothers Against Decapentaplegic Deleted in Pancreatic Carcinoma)

Anti -Smad3 (Small Mothers Against Decapentaplegic Deleted in Pancreatic Carcinoma)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IH IF
Catalog #S1014-53H1
ApplicationsSuitable for use in ELISA, Immunofluorescence, Western Blot and Immunohistochemistry. Other applications have not been tested.
Recommended Dilutions
Immunohistochemistry: 2.5ug/ml
Western Blot: 1:2000-1:20,000
ELISA: 1:25,000-1:100,000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide, KLH-conjugated. Epitope: Amino acids 417-425 of human SMAD3 protein.
SpecificityRecognizes Human SMAD, Mothers Against DPP Homolog 3 (SMAD3). Species Crossreactivity: Bovine, Chicken, Frog, Human, Mouse, Pig, Rat, Zebrafish.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesDKFZP586N0721, DKFZP686J10186, HMAD-3, HSMAD3, HSPC193, HST17436, JV15-2, MAD3, MADH3, MGC60396


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