| Catalog # | S1014-53H1 |
| Applications | Suitable for use in ELISA, Immunofluorescence, Western Blot and Immunohistochemistry. Other applications have not been tested. |
| Recommended Dilutions | |
| Immunohistochemistry: 2.5ug/ml |
| Western Blot: 1:2000-1:20,000 |
| ELISA: 1:25,000-1:100,000 |
| Optimal dilutions to be determined by the researcher. |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Host | Rabbit |
| Source | Human |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide. |
| Purity | Purified by immunoaffinity chromatography. |
| Immunogen | Synthetic peptide, KLH-conjugated. Epitope: Amino acids 417-425 of human SMAD3 protein. |
| Specificity | Recognizes Human SMAD, Mothers Against DPP Homolog 3 (SMAD3). Species Crossreactivity: Bovine, Chicken, Frog, Human, Mouse, Pig, Rat, Zebrafish. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
| Alternate names | DKFZP586N0721, DKFZP686J10186, HMAD-3, HSMAD3, HSPC193, HST17436, JV15-2, MAD3, MADH3, MGC60396 |