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You are here:Home » Antibodies » Abs to Smad Proteins » Anti -Smad3 (Small Mothers Against Decapentaplegic Deleted in Pancreatic Carcinoma)

Anti -Smad3 (Small Mothers Against Decapentaplegic Deleted in Pancreatic Carcinoma)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IH
Signaling events leading to transcriptional activation initiated by members of the TGF-beta superfamily are known to be mediated by SMAD proteins. Biological activities mediated by SMADs include cell growth and morphogenesis, development, and immune response. Activation by TGF-b is mediated by Smad2 and Smad3 while activation by bone morphogenetic protein (BMP) is mediated by Smad 1 and Smad 5. In contrast to the activating effects of these Smad proteins, Smad6 and Smad7, whose expression is also induced by ligand binding, appear to function as negative regulators of TGF-b superfamily signaling pathways.
Catalog #S1014-53J
ApplicationsSuitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications have not been tested.
Recommended DilutionELISA: 0.1-1ug/ml
Western Blot: 1-3ug/ml
Immunohistochemistry: 1ug/ml Requires HIER with EDTA, pH 8.0 prior to staining.
Optimal dilutions to be determined by the researcher.
Positive ControlsWestern Blot: human Smad3-transfected COS
Immunohistochemistry: human normal breast, esophagus, skin, and breast cancer tissues.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostRabbit
SourceHuman
Concentration~0.25mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.
PurityPurified by epitope affinity chromatography.
ImmunogenA 20 amino acid synthetic peptide derived from a central portion of the linker domain of human Smad3.
SpecificityRecognizes human Smad3. The antibody shows very minor cross-reactivity with Smad2 (using relatively high concentrations of recombinant Smad2).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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