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You are here:Home » Antibodies » Abs to Smad Proteins » Anti -Smad3 (Small Mothers Against Decapentaplegic Deleted in Pancreatic Carcinoma)

Anti -Smad3 (Small Mothers Against Decapentaplegic Deleted in Pancreatic Carcinoma)

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Supernatant B IP IH IC
Members of the SMAD family of signal transduction molecules are components of a critical intracellular pathway that transmits TGF-beta signals from the cell surface into the nucleus. Three distinct classes of SMADs have been defined: the receptor-regulated SMADs (R-SMADs), which include Smad1, 2, 3, 5 and 8, the common-mediator SMAD (co-SMAD), SMAD4, and the antagonistic or inhibitory SMADs (I-SMADs), SMAD6 and 7. Once in the nucleus, SMADs can target a variety of DNA binding proteins to regulate transcriptional responses. Following stimulation by TGF-beta, SMAD2 and SMAD3 become phosphorylated at their carboxyl termini (Ser465 and 467 on SMAD2; Ser423 and 425 on SMAD3) by TGF-beta Receptor I. Phosphorylated SMAD 2/3 can complex with SMAD4, translocate to the nucleus and regulate gene expression.
Catalog #S1014-53M
ApplicationsSuitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry, and Immunocytochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:5,000
Immunohistochemistry (paraffin): 1:100-250
Immunocytochemistry: 1:100-250
Immunoprecipitation: 1:50
Optimal dilutions to be determined by the researcher.
Positive ControlJurkat cell lysate
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG
Clone No9i210
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.01% sodium azide, 0.05% BSA, 40% glycerol.
PuritySupernatant
ImmunogenSynthetic peptide corresponding to an internal region of human SMAD3
SpecificityRecognizes human SMAD3. Species Crossreactivity: rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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