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You are here:Home » Antibodies » Abs to Smad Proteins » Anti -Smad3, phosphorylated (Ser423, Ser425) (Small Mothers Against Decapentaplegic Deleted in Pancreatic Carcinoma)

Anti -Smad3, phosphorylated (Ser423, Ser425) (Small Mothers Against Decapentaplegic
Deleted in Pancreatic Carcinoma)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
Smad3 is a ~50kD member of a family of proteins that act as key mediators of TGF- superfamily signaling in cell proliferation, differentiation and development. The Smad family is divided into three subclasses: receptor-regulated Smads, activin/TGF- receptor-regulated (Smad2 and 3) or BMP receptor regulated (Smad 1, 5 and 8); the common partner, (Smad4) that functions via its interaction to the various Smads; and the inhibitory Smads, (Smad6 and 7). Activated Smad3 oligomerizes with Smad4 upon TGF- stimulation and translocates as a complex into the nucleus, allowing its binding to DNA and transcription factors. Phosphorylation of the two TGF- -dependent serines 423 and 425 in the C-terminus of Smad3 is critical for Smad3 transcriptional activity and TGF- signaling.
Catalog #S1014-53P
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000
Optimal dilutions to be determined by the researcher.
Positive ControlNMuMG cells +/- TGF-
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in PBS (without Mg2+ and Ca2+), 1mg/ml BSA (IgG, protease free), pH 7.2, 0.05% sodium azide, 50% glycerol.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic phosphopeptide derived from a region of human Smad3 that contains serine 423 and serine 425.
SpecificityRecognizes human Smad3 when phosphorylated at Ser423 and Ser425. Species sequence homology: Mouse (100%). Does not cross-react with Smad2.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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