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Anti -Smad3, phosphorylated (Ser423, Ser425) (Small Mothers Against Decapentaplegic Deleted in Pancreatic Carcinoma)
Anti -Smad3, phosphorylated (Ser423, Ser425) (Small Mothers Against Decapentaplegic
Deleted in Pancreatic Carcinoma)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
B |
|
| Smad3 is a ~50kD member of a family of proteins that act as key mediators of TGF- superfamily signaling in cell proliferation, differentiation and development. The Smad family is divided into three subclasses: receptor-regulated Smads, activin/TGF- receptor-regulated (Smad2 and 3) or BMP receptor regulated (Smad 1, 5 and 8); the common partner, (Smad4) that functions via its interaction to the various Smads; and the inhibitory Smads, (Smad6 and 7). Activated Smad3 oligomerizes with Smad4 upon TGF- stimulation and translocates as a complex into the nucleus, allowing its binding to DNA and transcription factors. Phosphorylation of the two TGF- -dependent serines 423 and 425 in the C-terminus of Smad3 is critical for Smad3 transcriptional activity and TGF- signaling. | | | Catalog # | S1014-53P | | Applications | Suitable for use in Western Blot. Other applications not tested. | | Recommended Dilution | Western Blot: 1:1000 | | Optimal dilutions to be determined by the researcher. | | Positive Control | NMuMG cells +/- TGF- | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Polyclonal | | Host | Rabbit | | Source | Human | | Concentration | Not determined | | Form | Supplied as a liquid in PBS (without Mg2+ and Ca2+), 1mg/ml BSA (IgG, protease free), pH 7.2, 0.05% sodium azide, 50% glycerol. | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | Synthetic phosphopeptide derived from a region of human Smad3 that contains serine 423 and serine 425. | | Specificity | Recognizes human Smad3 when phosphorylated at Ser423 and Ser425. Species sequence homology: Mouse (100%). Does not cross-react with Smad2. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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