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You are here:Home » Antibodies » Abs to Smad Proteins » Anti -Smad3 (Small Mothers Against Decapentaplegic Deleted in Pancreatic Carcinoma)

Anti -Smad3 (Small Mothers Against Decapentaplegic Deleted in Pancreatic Carcinoma)

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Supernatant B IP IH IC
Smad proteins belong to a group of intracellular signal transducers and downstream effectors of TGF-beta/BMP Signaling. The family consists of nine members that are highly conserved in the N- and C-terminal regions (1). Smad3 is a key component in intracellular signaling of transforming growth factor beta (TGF-beta), an inhibitor for tumor cell proliferation (2). Smad3 is activated by activin/TGF-beta receptors, which form a heteromeric complex with Smad4, translocating to the nucleus and regulating the expression of TGF-beta target genes. Interaction of Smad3 with Akt, can initiate TGF-beta induced apoptosis and cell cycle arrest (3). Smad3 expression may be critical in tumor suppression in early stages of gastric carcinogenesis (4).
Catalog #S1014-53Q
ApplicationsSuitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000-1:10,000
Immunoprecipitation: 1:50
Immunohistochemistry: 1:100-1:250
Immunocytochemistry: 1:100-1:250
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
Clone TypeMonoclonal
IsotypeIgG
Clone No6k374
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.05% BSA, 0.01% sodium azide, 40% glycerol.
PuritySupernatant
ImmunogenSynthetic peptide corresponding to the internal region of human Smad3.
SpecificityRecognizes human Smad3 at ~58kD. Species Crossreactivity: mouse, rat
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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