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Anti -Smad3 (Small Mothers Against Decapentaplegic Deleted in Pancreatic Carcinoma)
Anti -Smad3 (Small Mothers Against Decapentaplegic Deleted in Pancreatic Carcinoma)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Rabbit |
Supernatant |
B IP IH IC |
|
| Smad proteins belong to a group of intracellular signal transducers and downstream effectors of TGF-beta/BMP Signaling. The family consists of nine members that are highly conserved in the N- and C-terminal regions (1). Smad3 is a key component in intracellular signaling of transforming growth factor beta (TGF-beta), an inhibitor for tumor cell proliferation (2). Smad3 is activated by activin/TGF-beta receptors, which form a heteromeric complex with Smad4, translocating to the nucleus and regulating the expression of TGF-beta target genes. Interaction of Smad3 with Akt, can initiate TGF-beta induced apoptosis and cell cycle arrest (3). Smad3 expression may be critical in tumor suppression in early stages of gastric carcinogenesis (4). | | | Catalog # | S1014-53Q | | Applications | Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested. | | Recommended Dilution | Western Blot: 1:1000-1:10,000 | | Immunoprecipitation: 1:50 | | Immunohistochemistry: 1:100-1:250 | | Immunocytochemistry: 1:100-1:250 | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG | | | Clone No | 6k374 | | Host | Rabbit | | Source | Human | | Concentration | Not determined | | Form | Supplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.05% BSA, 0.01% sodium azide, 40% glycerol. | | Purity | Supernatant | | Immunogen | Synthetic peptide corresponding to the internal region of human Smad3. | | Specificity | Recognizes human Smad3 at ~58kD. Species Crossreactivity: mouse, rat | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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