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You are here:Home » Antibodies » Abs to Smad Proteins » Anti -Smad3 (Small Mothers Against Decapentaplegic Deleted in Pancreatic Carcinoma)

Anti -Smad3 (Small Mothers Against Decapentaplegic Deleted in Pancreatic Carcinoma)

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Supernatant E B IP FC IF
Catalog #S1014-53R
ApplicationsSuitable for use in Immunofluorescence, Flow Cytometry, ELISA, Western Blot and Immunoprecipitation. Other applications not tested.
Recommended DilutionImmunofluorescence: 1:100
Flow Cytometry: 1:200
Western Blot: 1:1000
Immunoprecipitation: 1:100
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG
Clone No7H62
HostRabbit
SourceHuman
FormSupplied as a liquid in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100ug/ml BSA, 50% glycerol and less than 0.02% sodium azide.
PuritySupernatant.
ImmunogenSynthetic peptide (KLH-coupled) corresponding to residues at the amino terminus of Smad3.
SpecificityDetects endogenous levels of total Smad3 protein. No cross reactivity is detected with other family members. Cross-Reacitvity
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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