Anti -Smad3 (SMAD-3, DKFZP586N0721, DKFZp686J10186, hMAD-3, hSMAD3, HSPC193, HST17436,
JV15-2, MAD (Mothers Against Decapentaplegic Drosophila) Homolog 3, MAD3, MGC60396,
Mothers Against Decapentaplegic Homolog 3, Mothers Against DPP Homolog 3, MADH3, SMA and M
Pricing
For pricing information, USA customers sign in. Outside USA? Please contact your distributor for pricing. Specifications
| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Serum |
B IH |
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| Smad3 is a 50kD member of a family of proteins that act as key mediators of TGF beta superfamily signaling in cell proliferation, differentiation and development. The Smad family is divided into three subclasses: receptor regulated Smads, activin/TGF beta receptor regulated (Smad2 and 3) or BMP receptor regulated (Smad 1, 5, and 8); the common partner, (Smad4) that functions via its interaction to the various Smads; and the inhibitory Smads, (Smad6 and 7). Activated Smad3 oligomerizes with Smad4 upon TGF beta stimulation and translocates as a complex into the nucleus, allowing its binding to DNA and transcription factors. Phosphorylation of the two TGF beta dependent serines 423 and 425 in the C terminus of Smad3 is critical for Smad3 transcriptional activity and TGF beta signaling. | | | Catalog # | S1014-53U | | Applications | Suitable for use in Western Blot and Immunohistochemistry (paraffin-embedded sections). Other applications not tested. | | Recommended Dilution | Optimal dilutions to be determined by the researcher. | | Positive Control | Normal breast or breast carcinoma | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Host | Rabbit | | Source | Human | | Concentration | ~0.2mg/ml | | Form | BSA with 10mM PBS [pH 7.4] containing sodium azide | | Purity | Serum | | Immunogen | Synthetic peptide corresponding to internal region of human Smad3. | | Specificity | Species Crossreactivity: Reacts with Human, Mouse and Rat. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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Download Specifications for Anti -Smad3 (SMAD-3, DKFZP586N0721, DKFZp686J10186, hMAD-3, hSMAD3, HSPC193, HST17436, JV15-2, MAD (Mothers Against Decapentaplegic Drosophila) Homolog 3, MAD3, MGC60396, Mothers Against Decapentaplegic Homolog 3, Mothers Against DPP Homolog 3, MADH3, SMA and M |
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