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You are here:Home » Antibodies » Abs to Smad Proteins » Anti -SMAD3, phosphorylated (Ser208) (Mothers Against Decapentaplegic Homolog 3, SMAD 3, Mothers Against DPP Homolog 3, MAD Homolog 3, Mad3, hMAD-3, SMAD Family Member 3, JV15-2, hSMAD3, MADH3)

Anti -SMAD3, phosphorylated (Ser208) (Mothers Against Decapentaplegic Homolog 3, SMAD 3,
Mothers Against DPP Homolog 3, MAD Homolog 3, Mad3, hMAD-3, SMAD Family Member 3, JV15-2,
hSMAD3, MADH3)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E IF Gel
SMAD3, a receptor regulated SMAD (R-SMAD) is a transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinase. SMAD3 is estimated to account for at least 80% of all TGF-beta-mediated response. Activated type I receptor phosphorylates receptor-activated SMADS (RSMADS) at their c-terminal two extreme serines in the SSXS motif. The phosphorylated R-SMAD translocate into nucleus, where they regulate transcription of target genes. SMAD3 signal transduction appears to be important in the rgulation of muscle-specific genes. Loss of SMAD3 is a feature of pediatric T-cell lymphoblastic leukemia, while upregulation of SMAD3 may be responsible for TGFB hyperresponsiveness observed in scleroderma.
Catalog #S1014-54E1
ApplicationsSuitable for use in ELISA, Dot Blot and Immunofluorescence. Other applications not tested.
Recommended DilutionELISA: 1:1,000
Dot Blot: 1:500
Immunofluorescence: 1:10-1:50
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in PBS, 0.09% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic phosphopeptide corresponding to amino acid residues surrounding Ser208 of human SMAD3 (KLH).
SpecificityRecognizes human SMAD3 when phosphorylated at Ser208.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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