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You are here:Home » Antibodies » Abs to Smad Proteins » Anti -Smad4 (DPC4, Small Mothers Against Decapentaplegic Deleted in Pancreatic Carcinoma)

Anti -Smad4 (DPC4, Small Mothers Against Decapentaplegic Deleted in Pancreatic Carcinoma)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP IF
DPC4 (also named SMAD4; small mothers against decapentaplegic deleted in pancreatic carcinoma) belongs to the family of SMAD molecules comprising essential mediators of transforming growth factor beta (TGF-b) signalling. To date, seven members of this family have been identified, each of which plays a specific and separate role in mediating TGF-beta superfamily gene transcription. At least two different members, Smad2 and Smad4/DPC4, have been implicated in human cancer and appear to have tumor-suppressor functions whose inactivation may play an important role in the histogenesis of pancreatic and colorectal malignancy and possibly other human cancers as well.
Catalog #S1014-55
Applications and Suggested Dilutions
Immunofluorescence
Immunoprecipitation: Use Protein G at 2ug/mg protein lysate.
Western Blotting: 1ug/ml for 2hrs at RT
Optimal dilutions to be determined by the researcher.
Cellular LocalizationNuclear
Positive ControlLS174T, HT29, Raji, Jurkat cells or pancreatic and colorectal tumors.
HybridomaProduced by the fusion between BALB/c splenocytes and mouse myeloma NS2 cells.
Molecular Weight 60kD
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No0.N.557
HostMouse
SourceHuman
Concentration0.2mg/ml
FormSupplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 15mM sodium azide. Also available without BSA & Azide. See S1014-55B.
PurityPurified by Protein G affinity chromatography.
ImmunogenPurified recombinant DPC4 protein
SpecificityRecognizes human SMAD4/DPC4
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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