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You are here:Home » Antibodies » Abs to Smad Proteins » Anti -Smad4 (DPC4, Small Mothers Against Decapentaplegic Deleted in Pancreatic Carcinoma) (BSA & Azide Free)

Anti -Smad4 (DPC4, Small Mothers Against Decapentaplegic Deleted in Pancreatic Carcinoma)
(BSA & Azide Free)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP IF
DPC4 (also named SMAD4; small mothers against decapentaplegic deleted in pancreatic carcinoma) belongs to the family of SMAD molecules comprising essential mediators of transforming growth factor beta (TGF-b) signalling. To date, seven members of this family have been identified, each of which plays a specific and separate role in mediating TGF-beta superfamily gene transcription. At least two different members, Smad2 and Smad4/DPC4, have been implicated in human cancer and appear to have tumor-suppressor functions whose inactivation may play an important role in the histogenesis of pancreatic and colorectal malignancy and possibly other human cancers as well.
Catalog #S1014-55B
ApplicationsSuitable for use in Immunofluoresence, Immunoprecipitation, and Western Blot. Other applications not tested.
Recommended Dilutions:
Immunoprecipitation (Native and denatured): 2ug/mg protein lysate. Use Protein G .
Western Blot: 1-2ug/ml for 2hrs at RT
Optimal dilutions to be determined by the researcher.
Positive ControlLS174T, HT29, Raji and Jurkat cells
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No0.N.557
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in 10mM PBS, pH 7.4. Also available with BSA & Azide. See S1014-55.
PurityPurified by Protein G affinity chromatography.
ImmunogenPurified recombinant DPC4 protein. Molecular Weight
SpecificityRecognizes human SMAD4/DPC4
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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