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You are here:Home » Antibodies » Abs to Smad Proteins » Anti -Smad4, CT (DPC4, Small Mothers Against Decapentaplegic Deleted in Pancreatic Carcinoma)

Anti -Smad4, CT (DPC4, Small Mothers Against Decapentaplegic Deleted in Pancreatic
Carcinoma)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Supernatant B IP IH IC FC
SMAD4 is a member of the Mothers Against Dpp (MAD)-related family of proteins. SMAD4 is functionally distinct among the SMAD family, and is required for the assembly and transcriptional activation of diverse, SMAD-DNA complexes. SMAD4 appears to be not regulated by phosphorylation, but acts as a common mediator of TGF-beta, activin, and bone morphogenetic protein signaling responses. SMAD4 is frequently inactivated in pancreatic, biliary and colorectal tumors.
Catalog #S1014-55D
ApplicationsSuitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended DilutionsFlow Cytometry: 1:20
Western Blot: 1:1000-1:5000
Immunoprecipitation: 1:30
Immunohistochemistry (Paraffin): 1:100-250
Immunocytochemistry: 1:100-250
Optimal dilutions to be determined by the researcher.
Recommended Secondary Antibodies I1904-39 IgG, X-Adsorbed (HRP) Pab Gt xRb
I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb
I1904-46Q: IgG, H&L (HRP) Pab Gt xRb
Recommended Positive ControlSHSY5Y cell lysate
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG
Clone No9i212
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.05% BSA, 0.01% sodium azide, 40% glycerol.
PuritySupernatant
ImmunogenSynthetic peptide corresponding to residues in the C-terminus of human SMAD4.
SpecificityRecognizes human SMAD4 at ~65kD.
Species CrossreactivityMouse and rat
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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