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Anti -Smad5 phosphorylated (Ser463/465) (Small Mothers Against Decapentaplegic Deleted in Pancreatic Carcinoma)
Anti -Smad5 phosphorylated (Ser463/465) (Small Mothers Against Decapentaplegic Deleted in
Pancreatic Carcinoma)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Rabbit |
Supernatant |
B IC |
|
| Smad5 is a member of the Mothers Against Dpp (MAD)-related family of proteins. So far, eight Smads have been identified and can be divided into 3 subgroups based on their structure and function; pathway-restricted, common mediator and inhibitory. Smad 1, 5 and 8 serve as pathway-restricted Smads for the bone morphogenetic proteins (BMPs) signaling pathway (1). Smad5 is directly activated by BMP type Ia or Ib receptors upon BMP-2 stimulation and transduced BMP-2 signals to the nucleus by forming a complex with Smad 4, activating the transcription of the target gene in the nucleus (2-3). BMP activates Smad5 by phosphorylation at Ser463 and Ser465, which are the equivalent phosphorylation sites for Smad 1 and Smad 8. | | | Catalog # | S1014-55G3 | | Applications | Suitable for use in Immunocytochemistry, Western Blot. Other applications not tested. | | Recommended Dilution | Western Blot: 1:500 | | Immunocytochemistry: 1:50 -100 | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG | | Clone No | 9E553 | | Host | Rabbit | | Source | Human | | Concentration | Not determined | | Form | Supplied as a liquid in 50 mM Tris-Glycine, pH 7.4, 0.15 M sodium chloride, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. | | Purity | Supernatant | | Immunogen | A phospho specific peptide corresponding to residues surrounding Serine 463 and 465 of human Smad5. | | Specificity | Recognizes human Smad5 phosphorylated at Serine 463 and 465. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. | | Alternate names | SMAD 5, Mothers against DPP homolog 5, Smad5, hSmad5, JV5-1 |
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