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You are here:Home » Antibodies » Abs to Smad Proteins » Anti -Smad5 phosphorylated (Ser463/465) (Small Mothers Against Decapentaplegic Deleted in Pancreatic Carcinoma)

Anti -Smad5 phosphorylated (Ser463/465) (Small Mothers Against Decapentaplegic Deleted in
Pancreatic Carcinoma)

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Supernatant B IC
Smad5 is a member of the Mothers Against Dpp (MAD)-related family of proteins. So far, eight Smads have been identified and can be divided into 3 subgroups based on their structure and function; pathway-restricted, common mediator and inhibitory. Smad 1, 5 and 8 serve as pathway-restricted Smads for the bone morphogenetic proteins (BMPs) signaling pathway (1). Smad5 is directly activated by BMP type Ia or Ib receptors upon BMP-2 stimulation and transduced BMP-2 signals to the nucleus by forming a complex with Smad 4, activating the transcription of the target gene in the nucleus (2-3). BMP activates Smad5 by phosphorylation at Ser463 and Ser465, which are the equivalent phosphorylation sites for Smad 1 and Smad 8.
Catalog #S1014-55G3
ApplicationsSuitable for use in Immunocytochemistry, Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1:500
Immunocytochemistry: 1:50 -100
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
Clone TypeMonoclonal
IsotypeIgG
Clone No9E553
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in 50 mM Tris-Glycine, pH 7.4, 0.15 M sodium chloride, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
PuritySupernatant
ImmunogenA phospho specific peptide corresponding to residues surrounding Serine 463 and 465 of human Smad5.
SpecificityRecognizes human Smad5 phosphorylated at Serine 463 and 465.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesSMAD 5, Mothers against DPP homolog 5, Smad5, hSmad5, JV5-1


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