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You are here:Home » Antibodies » Abs to Smad Proteins » Anti -Smad7 (Small Mothers against Decapentaplegic Deleted in Pancreatic Carcinoma)

Anti -Smad7 (Small Mothers against Decapentaplegic Deleted in Pancreatic Carcinoma)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH
Smads are a family of intracellular proteins that transmit transforming growth factor beta (TGF-b) superfamily signals from the cell surface to the nucleus. The Smad family is divided into three subclasses: receptor regulated Smads, (Smads 1, 2, 3, 5 and 8); the common partner, (Smad4) that functions via its interaction to the various Smads; and the inhibitory Smads, (Smads 6 and 7). SMAD7, also known as Mothers Against Decapentaplegic homolog 7 (MADH7), inhibits selected pathways by binding directly to cell-surface receptors and preventing the activation-induced phosphorylation of other Smad subunits.
Catalog #S1014-55K
ApplicationsSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionsWestern Blot: 1ug/ml
Immunohistochemistry: 8-25ug/ml with appropriate secondary reagents to detect SMAD7 in paraffin-embedded human liver cancer tissue sections.
Optimal dilutions to be determined by the researcher.
Recommended Secondary AntibodiesI1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo
I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo
HybridomaMouse myeloma cells with B cells from BALB/c mice.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. 
Clone TypeMonoclonal
IsotypeIgG2b
Clone No7H261
HostMouse
SourceHuman
Concentration~0.5mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
PurityPurified by Protein G affinity chromatography.
ImmunogenRecombinant protein corresponding to aa320-398 from human Smad7, expressed in E. coli (#O15105).
SpecificityRecognizes endogenous levels of human SMAD7.
Species CrossreactivityMouse and rat
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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