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Anti -Smad4, CT (Small Mothers Against Decapentaplegic Deleted)
Anti -Smad4, CT (Small Mothers Against Decapentaplegic Deleted)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Rabbit |
Supernatant |
B IP IH IC FC |
|
| Smad 4 is a member of the Mothers Against Dpp (MAD)-related family of proteins. So far, eight Smads have been identified and can be divided in 3 subgroups based on their structure and functions; pathway-restricted, common mediator and inhibitory Smad. Smad 4 is the common Smad (co-Smad) (1-2). Previously identified as the tumor suppressor DPC4 (deleted in pancreatic carcinoma, locus 4), Smad 4 is functionally distinct among the Smad family, and is required for the assembly and transcriptional activation of diverse, Smad-DNA complexes (3). In contrast to the R-Smads, Smad 4 is not regulated by phosphorylation, but acts as a common mediator of TGF- , activin, and bone morphogenetic protein signaling responses (4). Smad 4 is frequently inactivated in pancreatic, biliary and colorectal tumors (5-6). | | | Catalog # | S1014-55P | | Applications | Suitable for use in Flow Cytometry, Western Blotting, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry. Other applications not tested. | | Recommended Dilution | Flow Cytometry: 1:20 | | Western Blotting: 1:1,000-5,000 (65kD) | | Immunohistochemistry: 1:100-250 | | Immunocytochemistry: 1:100-250 | | Immunoprecipitation: 1:30 1ug/mg of total cell protein (~10e7 cells) | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG | | Clone No | 6k375 | | Host | Rabbit | | Source | Human | | Concentration | Not determined | | Form | Supplied as a liquid. | | Purity | Supernatant | | Immunogen | A synthetic peptide corresponding to residues in the C-term of human Smad 4. | | Specificity | Recognizes human Smad 4. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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