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You are here:Home » Antibodies » Abs to Smad Proteins » Anti -Smad4, CT (Small Mothers Against Decapentaplegic Deleted)

Anti -Smad4, CT (Small Mothers Against Decapentaplegic Deleted)

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Supernatant B IP IH IC FC
Smad 4 is a member of the Mothers Against Dpp (MAD)-related family of proteins. So far, eight Smads have been identified and can be divided in 3 subgroups based on their structure and functions; pathway-restricted, common mediator and inhibitory Smad. Smad 4 is the common Smad (co-Smad) (1-2). Previously identified as the tumor suppressor DPC4 (deleted in pancreatic carcinoma, locus 4), Smad 4 is functionally distinct among the Smad family, and is required for the assembly and transcriptional activation of diverse, Smad-DNA complexes (3). In contrast to the R-Smads, Smad 4 is not regulated by phosphorylation, but acts as a common mediator of TGF- , activin, and bone morphogenetic protein signaling responses (4). Smad 4 is frequently inactivated in pancreatic, biliary and colorectal tumors (5-6).
Catalog #S1014-55P
ApplicationsSuitable for use in Flow Cytometry, Western Blotting, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry. Other applications not tested.
Recommended DilutionFlow Cytometry: 1:20
Western Blotting: 1:1,000-5,000 (65kD)
Immunohistochemistry: 1:100-250
Immunocytochemistry: 1:100-250
Immunoprecipitation: 1:30 1ug/mg of total cell protein (~10e7 cells)
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
Clone TypeMonoclonal
IsotypeIgG
Clone No6k375
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid.
PuritySupernatant
ImmunogenA synthetic peptide corresponding to residues in the C-term of human Smad 4.
SpecificityRecognizes human Smad 4.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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