Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Antibodies » Abs to Smad Proteins » Anti -SMAD9 (Mothers Against Decapentaplegic Homolog 9, MAD Homolog 9, Mothers Against DPP Homolog 9, Madh6, SMAD Family Member 9, SMAD 9, Smad9, MADH6, MADH9)

Anti -SMAD9 (Mothers Against Decapentaplegic Homolog 9, MAD Homolog 9, Mothers Against DPP
Homolog 9, Madh6, SMAD Family Member 9, SMAD 9, Smad9, MADH6, MADH9)

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications
Polyclonal Sheep Affinity Purified IH IC
SMAD9 is a 55-60kD R-Group member of the dwarfin/SMAD family of proteins. It is expressed by BMP-responsive cells, and serves to transmit information from the BMP receptor (BMPR) to the nucleus. In particular, following BMPR activation, SMAD9 is phosphorylated by the BMPR on its C-terminus, promoting heterodimerization with SMAD4 and its translocation into the nucleus. Here, SMAD9 binds to DNA and participates in gene activation. Human SMAD9 is 467aa in length. It contains a DNA-binding domain termed MH1 aa16-140 plus a receptor/transcription factor binding domain termed MH2 aa273-467. The C-terminus contains two phosphorylation sites at aa465 and aa467. There is one splice form that shows a deletion of aa224-260. Over aa158-223, human and mouse SMAD9 share 82% identy in aa sequence.
Catalog #S1014-55Q
ApplicationsSuitable for use in Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended DilutionImmunohistochemistry: 5-15ug/ml
Immunocytochemistry: 5-15ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostSheep
SourceHuman
Concentration~0.1mg/ml after reconstitution
FormSupplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.2mg/ml.
PurityPurified by immunoaffinity chromatography.
ImmunogenRecombinant corresponding to aa158-223 from human SMAD9 expressed in E.coli.
SpecificityRecognizes human SMAD9.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links