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Specifications

Clone Host Grade Applications Monoclonal Mouse Purified B IH

Smoothelin is a constituent of the smooth muscle cell (SMC) cytoskeleton. Antibodies directed to smoothelin comprise a useful tool to monitor SMC differentiation. Smoothelin is exclusively expressed in fully differentiated (contractile) SMCs. RNA and protein analyses revealed a broad species distribution of this protein. Cells with SMC-like characteristics, such as myofibroblasts and myoepithelial cells as well as skeletal and cardiac muscle, do not contain smoothelin. Confocal scanning laser microscopy of tissue sections show a filamentous organization of smoothelin, colocalizing with actin stress fibers. In immunoblots two molecular weight isoforms are detected, i.e., a 59kD isoform specific for visceral SMC (Smoothelin A) and an isoform with a molecular weight of ~100kD in vascular SMC (Smoothelin B). Human smoothelin is encoded by a single copy gene which is located on chromosome 22. The monoclonal smoothelin antibody is useful for discriminating fully differentiated, contractile SMCs from proliferative SMCs. The distribution of smoothelin-positive cells indicates a correlation between smoothelin expression and smooth muscle tissue contractility. Smoothelin is a useful tool in the evaluation of atherosclerotic lesions.
Catalog #	S1014-62A
Applications	Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilutions	Western Blot: 1:100-1:1000
	Immunohistochemistry (Frozen/paraffin): 1:100-1:200, using ABC as detection reagent. For paraffin sections, pretreatment in 10mM citrate buffer, pH 6.0 and heating 3 times for 5 minutes in a microwave is required.
	Optimum dilutions to be determined by researcher.
Hybridoma	Sp2/0-AG14 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	LgG1
Clone No	4A83
Host	Mouse
Source	Chicken
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity	Purified
Immunogen	Cytoskeletal extract of chicken gizzard.
Specificity	Recognizes chicken 59kD and 100kD protein, corresponding to smoothelin A and B, respectively, which are exclusively found in smooth muscle cells. Species Crossreactivity: human, canine, feline, porcine and monkey. Not reactive with rat.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.