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You are here:Home » Antibodies » Abs to Enzymes, Phospholipase » Anti -SMPD1, ID (Lyso-platelet-activating Factor-phospholipase C, Neutral Sphingomyelinase, nSMase, N-SMase, Sphingomyelin Phosphodiesterase 1, Lyso-PAF-PLC)

Anti -SMPD1, ID (Lyso-platelet-activating Factor-phospholipase C, Neutral
Sphingomyelinase, nSMase, N-SMase, Sphingomyelin Phosphodiesterase 1, Lyso-PAF-PLC)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E IP N
SMPD1 (Sphingomyelin phosphodiesterase 1; also ASM) is a 70-72kD member of the sphingomyelinase family of enzymes. It is a monomeric lysosomal enzyme that converts sphingomyelin (a plasma membrane lipid) into ceramide through the removal of phosphorylcholine. This generates second messenger components that participate in signal transduction. Mature human SMPD1 is 583aa in length. It contains one saposin B-type domain (aa85-169) that likely interacts with sphingomyelin, and a catalytic region (aa 267-363). There are multiple isoforms. One is 57kD in size and results from proteolytic cleavage between Pro179-Pro197. Three others show aa substitutions; a 56aa substitution for (aa1-361), a two aa substitution for (aa363-629), a12aa substitution for (aa363-418). Over (aa62-628), human SMPD1 is 86% aa identical to mouse SMPD1.
Catalog #S1014-63S
ApplicationsSuitable for use in ELISA, Immunoprecipitation, Neutralization.. Other applications not tested.
Recommended DilutionDirect ELISA: 0.5-1ug/ml
Immunoprecipitation: 0.5-1ug/ml
Neutralization: 0.5ug/ml
Optimal dilutions to be determined by the researcher.
HybridomaMyeloma cells with B cells from Balb/c mice.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No11D15
HostMouse
SourceHuman
Concentration~0.5mg/ml (after reconstitution)
FormSupplied as a lyophilized powder in PBS with 5% trehalose. Reconstitute with 200ul sterile PBS.
PurityPurified by Protein G affinity chromatography.
ImmunogenRecombinant corresponding to aa62-628 of human SMPD1 expressed in CHO cells (NP_000534).
SpecificityRecognizes human SMPD1.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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