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Specifications

Clone Host Grade Applications Polyclonal Goat Affinity Purified E B

This gene encodes a member of the sorting nexin family. Members of this family contain a phox (PX) domain, which is a phosphoinositide binding domain, and are involved in intracellular trafficking. This protein binds to fanconi anemia complementation group A protein, but its function is unknown. This gene results in two transcript variants encoding the same protein.
Catalog #	S1014-97F2
Applications	Suitable for use in ELISA and Western Blot. Other Applications not tested.
Recommended Dilutions	ELISA: 1:32,000
	Western Blot: 1-3ug/ml
	Optimal dilutions to be determined by the researcher
Positive Control	Human Kidney, Jurkat, and A549 lysates
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 monthsat -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Peptide Blocking	Corresponding peptide is available for Peptide Blocking studies. See S1014-97F2-P. Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet. When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml. To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Goat
Source	Human
Concentration	~0.5mg/ml
Form	Supplied as a liquid in Tris, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Purity	Purified by affinity chromatography.
Immunogen	Synthetic peptide, SLLQSCIDLFKNN, from the C-Terminus of human SNX5.
Specificity	Recognizes human SNX5 at ~48kD. Species sequence homology: mouse, rat, canine.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.