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S1030 SOC Broth (Powder)

Specifications
References
Grade
Molecular Biology Grade
EU Commodity Code
38210000
Shipping Temp
RT
Storage Temp
RT/4°C
Tryptone, Yeast Extract, Sodium Chloride, MgSO4•7H2O, Dextrose•H2O

SOC Broth is a rich media used primarily in the recovery step of Escherichia coli competent cell transformations. Use of SOC Broth maximizes the transformation efficiency of competent cells.

BSE-Free
All raw materials of animal origin are sourced from USDA-designated BSE-Free countries
Dust-Free
Specially milled powder blends are designed to eliminate or greatly reduce airborne particulates
Homogeneous
Proprietary blending technology ensures complete mixing of components
Appearance
Light tan, homogeneous, free flowing powder
Solubility
Light yellow, clear, complete
pH
6.8-7.2
Directions per Liter
Dissolve 34.46g in 800-900ml of ddH2O stirring gently with heating until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to 1L. Sterilize with 0.2um membrane filter. Aliquot into sterile containers.
Note: This solution can be autoclaved; extended temperatures could cause the solution to darken, which will not affect product usefulness.
Storage and Stability
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.
Media Formulation
Components shown as g/Liter
Dextrose•H2O [20mM]3.96
MgSO4•7H2O5
Sodium Chloride0.5
Tryptone20
Yeast Extract5
Total: 34.46g/L
References
1. Sambrook, J., et al., Molecular Cloning: A Laboratory Manual 2nd ed., Plainview, NY (1989), 1.76-1.81& A.2.
USBio References
No references available
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