sodium-independent neutral amino acid transporter LAT1, large neutral amino acids transporter small subunit 1, large neutral amino acids transporter 1, integral membrane protein E16, L-type amino acid transporter 1, CD98 light chain, 4F2 light chain, hLAT1, MPE16, LAT1, E16, D16S469E, CD98, 4F2LC, solute carrier family 7 (cationic amino acid transporter, y+ system), member 5, SLC7A5
Applications
Suitable for use in ELISA and Western Blot. Other applications have not been tested.
Recommended Dilutions
Peptide ELISA Titer: 1:64,000 Western Blot: 1-3ug/ml detects a ~60kD band observed in Human Spleen lysates (calculated MW of 55.0kD according to NP_003477.4). Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to C-RHRKPELERPIKVN, from the internal region of human SLC7A5 (NP_003477.4).
Form
Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.
Purity
Purified by peptide affinity chromatography.
Specificity
Recognizes human SLC7A5. Species sequence homology: canine.
References
1.Ohno Y, Suda K, Masuko K, Yagi H, Hashimoto Y, Masuko T. Production and characterization of highly tumor-specific rat monoclonal antibodies recognizing the extracellular domain of human L-type amino-acid transporter 1. Cancer Sci. 2008 May;99(5):1000-7.