Anti -Spectrin alpha chain, erythrocyte (Spectrin, alpha, erythrocytic 1, EL2, Erythroid
alpha-spectrin, HPP, HS3, Spectrin alpha chain, erythrocyte, SPH3, SPTA)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Purified |
E B IH IF |
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| Spectrin is the predominant component of the membrane skeleton of the red blood cell and is essential in determining shape and deformability of the membrane. It consists of 2 nonidentical subunits, alpha (MW 240,000) and beta (MW 225,000). Spectrin is present in the red cell membrane in a tetrameric or possibly higher polymeric form through head to head self association of heterodimers that are linked by actin polymers and protein 4.1 to form a 2 dimensional network. | | | Catalog # | S5381-03C | | Applications | Suitable for use in Immunofluorescence, ELISA, Western Blot, and Immunohistochemistry. Other applications not tested. | | Recommended Dilution | Immunofluorescence: 1:100 -1:500 | | ELISA: 1:105-1:106 | | Western Blot: 1:102-1:103 | | Immunohistochemistry: 1:10-1:50 | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG1 | | Clone No | 9L188 | | Host | Mouse | | Concentration | ~1mg/ml | | Form | Supplied as a liquid in PBS, 0.1% sodium azide. | | Purity | Purified by ammonium sulfate precipitation and dialysis. | | Immunogen | Recombinant alpha I spectrin (erythroid spectrin) SH3 domain | | Specificity | Recognizes alpha I spectrin SH3 domain. Does not cross react with alpha II (non-erythroid) spectrin SH3 domain or recombinant human fodrin SH3 domain. Species Crossreactivity: human, rat, mouse | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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Download Specifications for Anti -Spectrin alpha chain, erythrocyte (Spectrin, alpha, erythrocytic 1, EL2, Erythroid alpha-spectrin, HPP, HS3, Spectrin alpha chain, erythrocyte, SPH3, SPTA) |
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