| Spermine, a polyamine, is a positively charged compound found in living cells and can cause conformational changes in DNA via charge and solubility mechanisms which can affect gene expression cell differentiation. Spermine may be linked to the development of cancer. |
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| Catalog # | S5390 |
| Applications | |
| Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. |
| Recommended Dilution | ELISA: 1:2000-1:5000 |
| Immunohistochemistry: 1:500- 1:1000 |
| Western Blot: 1:1000- 1:2000 |
| Optimal dilutions to be determined by the researcher. |
| Specificity | Using a conjugate Spermine- Glutaric Anhydride-Protein, antibody specificity was performed by competition ELISA with the following compounds: |
| Compound Cross-reactivity ratio Spermine-GA-BSA 1 Spermidine-GA-BSA 1 | Diamino-Pentane-G-BSA 1:700 |
| Ornithine-GA-BSA 1:500 |
| G = Glutaraldehyde, GA = Glutaric Anhydride |
| Control Peptide | S5390-01 |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.2, 40% glycerol. Preservative free. |
| Purity | Antiserum previously preabsobed on protein carriers, and purified by ammonium sulfate precipitation and gel filtration. |
| Immunogen | Synthetic Spermine-Glutaric Anhydride-BSA. |
| Specificity | Recognizes protein-conjugated Spermine. See additional crossreactivity data. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
