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You are here:Home » Antibodies » Abs to Splicing Factors » Anti -SF3B4 (Splicing Factor 3B Subunit 4, Pre-mRNA-splicing Factor SF3b 49kD Subunit, SF3b50, Spliceosome-associated Protein 49, SAP 49, SAP49)

Anti -SF3B4 (Splicing Factor 3B Subunit 4, Pre-mRNA-splicing Factor SF3b 49kD Subunit,
SF3b50, Spliceosome-associated Protein 49, SAP 49, SAP49)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B
The SF3B4 gene encodes one of four subunits of the splicing factor 3B. The protein encoded by this gene cross-links to a region in the pre-mRNA immediately upstream of the branchpoint sequence in pre-mRNA in the prespliceosomal complex A. It also may be involved in the assembly of the B, C and E spliceosomal complexes. In addition to RNA-binding activity, this protein interacts directly and highly specifically with subunit 2 of the splicing factor 3B. This protein contains two N-terminal RNA-recognition motifs (RRMs), consistent with the observation that it binds directly to pre-mRNA.
Catalog #S5555-50J
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionELISA: 1:32,000
Western Blot: 0.1-0.3ug/ml, observed in human HeLa lysates on ~48kD bands
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
HostGoat
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to AAGPISERNQDAT-C, from human SF3B4, at N-terminal (NP_005841.1).
SpecificityRecognizes human SF3B4. Species sequence homology: Bovine, canine, mouse, porcine and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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