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You are here:Home » Antibodies » Abs to Sprouty » Anti -Sprouty (Spry)

Anti -Sprouty (Spry)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP
Sprouty is a suppressor of EGF and FGF signaling that appears to have a universal inhibitory role on tyrosine kinase signaling. Evidence suggests that it does this by binding adapter proteins and GAP, thus inactivating Ras. Sprouty expression is induced by the same pathways that it down-regulates, suggesting it plays a role in an auto-feedback loop.
Catalog #S5620
Sprouty was originally identified as an inhibitor of Drosophila epidermal growth factor (EGF) and fibroblast growth factor (FGF) receptor signaling during tracheal development. Four isoforms of mammalian sprouty are known (Spry1-Spry4). The role of the mammalian analogs has not been clearly elucidated although it is believed that human Sprouty-2 (hSpry2) may be an inhibitor of cellular migration and proliferation. Spry2 and Spry4 show considerable sequence homology between human and mouse at the C-terminus of the protein. Significant sequence divergence occurs at the N-terminus. Spry2 appears to be abundant in brain, lung and heart tissue, with lesser amounts found in kidney and skeletal muscle.
ApplicationsSuitable for use in Western Blotting, Immunoprecipitation. Other applications not tested.
Recommended DilutionWestern Blot : 0.5-2ug/ul. Transfected Cos-7 cell lysate probed with anti-Spry (0.5ug/ul).
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
FormSupplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide before the addition of 30% glycerol.
PurityPurified by Protein A affinity chromatography.
ImmunogenHis-Tagged fusion protein corresponding to residues 1-207 of human Sprouty.
SpecificityRecognizes Sprouty, Mr 35-40kDa. Species Crossreactivity: Human.
The immunogen is
100% homologous with human Spry 2
45% homologous with human Spry 1
44% homologous with human Spry 4
31% homologous with human Spry 3
43% homologous with mouse Spry 4
Important NoteThis product is intended for research use only, not for use in human, therapeutic or diagnostic applications.


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