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You are here:Home » Antibodies » Abs to Sprouty » Anti -Sprouty 1 (Spry 1)

Anti -Sprouty 1 (Spry 1)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
Sprouty-1 and -2 are membrane-anchored phosphoprotein inhibitors of growth factor signaling in endothelial cells. Four mammalian Sprouty homologues (Sprouty-1–4) have been identified. One study showed that overexpressed Sprouty-1 and -2 inhibits fibroblast growth factor– and vascular endothelial growth factor–induced proliferation and differentiation by repressing
pathways leading to MAP kinase activation.1 Sprouty proteins are also endogenous inhibitors of the Ras/MAP kinase pathway that play an important role in the remodeling of branching tissues, such as in development of the lung, kidney tubules, vascular system, and breast ducts.
A recent study shows that Sprouty-1 and –2 may have tumor suppressing activity in the breast, and found that both proteins are consistently down-regulated in breast carcinomas.
Catalog #S5620-01B
ApplicationsSuitable for use in Western Blot. Other applications have not been tested.
Recommended DilutionWestern Blot: 1-3ug/ml
Optimal dilution to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostRabbit
SourceMouse
Concentration~0.25mg/ml
FormSupplied as a liquid in PBS, 0.1% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide derived from the N-terminal region of mouse and rat Sprouty-1
SpecificityRecognizes mouse Sprouty-1 at ~34kD. A band of unknown origin was observed at ~120kD. Reactivity has been confirmed with two day-old mouse kidney homogenates. Does not recognize rat Sprouty-1.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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