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You are here:Home » Antibodies » Abs to Sprouty » Anti -Sprouty Homolog 1 (Protein Sprouty Homolog 1, Spry-1, SPRY1)

Anti -Sprouty Homolog 1 (Protein Sprouty Homolog 1, Spry-1, SPRY1)

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Specifications

Clone Host Grade Applications
Polyclonal Sheep Affinity Purified B
SPRY1 (sprouty homolog 1) is a 34-38kD member of the sprouty family of proteins. It is widely expressed, being found in multiple embryonic and adult tissues. SPRY1 is considered a negative regulator of cellular signaling. In particular, it appears to both inhibit MAP kinase signaling following RTK activation, and block TCR signaling following antigen activation. It interacts with a number of molecules, including PLC-gama-1, LAT, CBL, caveolin-1 and SPRY2. Human SPRY1 is 319 aa in length and contains one CBL-TKB binding site (aa51-57) that is phosphorylated at Tyr53, a Ser-rich region (aa112-131), and a Cys-rich domain (aa181-306) that mediates intracellular translocation. In addition, SPRY1 undergoes serine phosphorylation, ubiquitination and palmitoylation, the latter which induces SPRY1 to associate with cell membranes.
Catalog #S5620-01D
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionsWestern Blot: 1ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS, 0.02% NaN3. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostSheep
SourceHuman
ConcentrationNot determined
FormSupplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with PBS, 0.02% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenE.coli-derived recombinant human SPRY1 (aa1-178; Accession # O43609).
SpecificityRecognizes human SPRY1.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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