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You are here:Home » Antibodies » Abs to Sprouty » Anti -Sprouty 2 (SPRY2, Protein Sprouty Homolog 2, Spry-2)

Anti -Sprouty 2 (SPRY2, Protein Sprouty Homolog 2, Spry-2)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified B
SPRY2 (sprouty homolog 2) is a 35kD member of the sprouty family of proteins. It is widely expressed in embryonic and adult tissues, including endothelium. SPRY2 is considered a negative regulator of Ras/ERK signaling. For example, it appears to form a trimeric complex with PKC-delta and PKD1, blocking ERK phosphorylation. It also interacts with a number of other molecules, including CBL and SIAH2, which likely limit its availability. Human SPRY2 is 315 aa in length and contains one CBL-TKB binding site (aa53-59) that is phosphorylated at Tyr55, a Ser-rich region (aa125-131), a Cys-rich domain (aa178-301) and a PxxPxR motif that mediates ERK inhibitory activity.
Catalog #S5620-02D
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionsWestern Blot: 1ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS, 0.02% sodium azide. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
ConcentrationNot determined
FormSupplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with sterile PBS, 0.02% sodium chloride.
PurityPurified by immunoaffinity chromatography.
ImmunogenPurified, E. coli-derived recombinant human SPRY2 (rhSPRY2; aa1-175; Accession # O43597).
SpecificityRecognizes human SPRY2. Species Crossreactivity: mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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