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Anti -Sprouty 2 (SPRY2, Protein Sprouty Homolog 2, Spry-2)
Anti -Sprouty 2 (SPRY2, Protein Sprouty Homolog 2, Spry-2)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Goat |
Affinity Purified |
B |
|
| SPRY2 (sprouty homolog 2) is a 35kD member of the sprouty family of proteins. It is widely expressed in embryonic and adult tissues, including endothelium. SPRY2 is considered a negative regulator of Ras/ERK signaling. For example, it appears to form a trimeric complex with PKC-delta and PKD1, blocking ERK phosphorylation. It also interacts with a number of other molecules, including CBL and SIAH2, which likely limit its availability. Human SPRY2 is 315 aa in length and contains one CBL-TKB binding site (aa53-59) that is phosphorylated at Tyr55, a Ser-rich region (aa125-131), a Cys-rich domain (aa178-301) and a PxxPxR motif that mediates ERK inhibitory activity. | | | Catalog # | S5620-02D | | Applications | Suitable for use in Western Blot. Other applications not tested. | | Recommended Dilutions | Western Blot: 1ug/ml | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS, 0.02% sodium azide. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Goat | | Source | Human | | Concentration | Not determined | | Form | Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with sterile PBS, 0.02% sodium chloride. | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | Purified, E. coli-derived recombinant human SPRY2 (rhSPRY2; aa1-175; Accession # O43597). | | Specificity | Recognizes human SPRY2. Species Crossreactivity: mouse. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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