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You are here:Home » Antibodies » Abs to Sprouty » Anti -Sprouty 3 (SPRY3, Sprouty homolog 3 (Drosophila) HSPRY3, Sprouty homolog 3, Spry-3)

Anti -Sprouty 3 (SPRY3, Sprouty homolog 3 (Drosophila) HSPRY3, Sprouty homolog 3, Spry-3)

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Specifications

Clone Host Grade Applications
Polyclonal Sheep Affinity Purified B
SPRY3 (sprouty homolog 3) is a member of the sprouty family of proteins. SPRY3 appears to modulate Ras/MAPK pathway signaling following RTK activation. Human SPRY3 is 288aa in length and contains one SH2-binding domain (aa 25-30), a PEST sequence (aa82-93), and a Cys-rich domain (aa147-270). SPRY3 forms homo- and hetero-oligomers with other SPRY family molecules. It also undergoes phosphorylation, ubiquitination and palmitoylation, the latter of which induces SPRY3 to associate with cell membranes.
Applications:
Suitable for use in Western Blot. Other applications not tested.
Catalog #S5620-04
Recommended DilutionWestern Blot: 1.0ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile 40-50% glycerol, PBS.. Aliquot and store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostSheep
SourceHuman
ConcentrationAs reported
FormSupplied as a lyophilized powder in 0.2um sterile-filtered solution, PBS,5% trehalose. Reconstitute with 40-50% glycerol, PBS.
PurityPurified by immunoaffinity chromatography.
ImmunogenRecombinant human SPRY3 aa1-142; O43610 (E. coli).
SpecificityRecognizes human SPRY3 at ~42kD.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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