Anti -Sprouty 4 (Sprouty-4, Sprouty Homolog 4, Spry 4, SPRY4, SPRY-4)
Pricing
For pricing information, USA customers sign in. Outside USA? Please contact your distributor for pricing. Specifications
| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
E B IH |
|
| Sprouty-4 (also known as SPRY4) is an inhibitor of the insulin receptor and EGFR-transduced mitogen-activated protein kinase (MAPK) signaling pathway downstream of FGF and EGF receptor tyrosine kinase activation. It is positioned upstream of RAS activation and impairs the formation of active GTP-RAS. SPRY4 is widely expressed, with different isoforms. The protein consists of 322 amino acids, with a cysteine-rich region, Src homology 3 binding regions, proline-rich regions, and a PEST sequence. It is expressed predominantly in the cytoplasm. Northern results show bands across most tissues, with strongest expression in heart, brain, placenta, lung, and intestine. | | | Catalog # | S5620-25A | | Applications | Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. | | Recommended Dilution | Optimal dilutions to be determined by the researcher. | | Cellular Localization | Cytoplasmic | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Human | | Concentration | ~0.93mg/ml | | Form | 0.15M Sodium Chloride, 0.02M Potassium Phosphate [pH 7.2] | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | Synthetic peptide with sequence CKAASGDAKTSRPDKPF conjugated to KLH, corresponding to amino acids 283-299 of Human Sprouty 4. Epitope: aa 283-299 | | Specificity | Species Crossreactivity: Expected to crossreact with Human (100% identity with immunogen) and Mouse (88% identity with immunogen) due to sequence homology. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
External Links
|
