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You are here:Home » Antibodies » Antibodies-Protein Kinase Substrates » Anti -Src (pp60Src, pp60c-Src)

Anti -Src (pp60Src, pp60c-Src)


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Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP
Src is a member of the Src family of protein tyrosine kinases. This kinase is expressed in different tissues of the body with the highest protein levels detected in neurons and platelets. Src can modulate the signal transduction pathways activated by several growth factors (e.g., PDGF, M-CSF and G-CSF) and integrins. Src also regulates the activity of several ion channels including the N-methyl-D-aspartate (NMDA) receptor. In addition, Src is thought to play a role in physiological/pathophysiological processes in the central nervous system. It has also been reported that mice deficient in Src develop severe osteoporosis. A total of eight membrane-associated protein tyrosine kinases have been identified within the Src gene family.
Catalog #S6500
ApplicationsSuitable for use in Immunoprecipitation and Western Blot analysis. Other applications have not been tested.
Recommended DilutionImmunoprecipitation (kinase): 4ug; Immunoprecipitates Src from 500ug of A431 RIPA lysate.
Western Blot: 0.5-2mg/ml. Detects Src in RIPA lysates from non-stimulated A431 carcinoma cells. Previous lots detected Src in RIPA lysates from EGF-stimulated A431 cells, mouse 3T3/A31 cells and Cos-1 cells transfected with pUSE Mouse Src.
Optimal dilutions to be determined by the researcher.
Positive Control IncludedA0001-50: A431 Cells, Non-Stimulated in RIPA Buffer, Sample Buffer. Add 2.5ul of 2-mercapoethanol per 100ul of lysate and boil for 5 minutes. Use 20ug per lane for minigels.
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
Clone No0.T.137
FormSupplied as a liquid in PBS, pH 7.3, 0.005M EDTA, 0.05% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenRecombinant chicken pp60Src purified from extracts of E. coli containing the plasmid plac-Src. Epitope is mapped to aa82-169.
SpecificityRecognizes pp60Src at 60kD. Species Crossreactivity: Human, mouse, rat, mink and avian species.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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