| The Src family of protein tyrosine kinases including Src, Lyn, Fyn, Yes, Lck, Blk, Hck, etc.) are |
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| important in the regulation of growth and differentiation of eukaryotic cells (1). Src activity is regulated by tyrosine phosphorylation at two sites with opposing effects. Phosphorylation of Tyr416 in the activation loop of the kinase domain upregulates enzyme activity. Phosphorylation |
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| of Tyr527 in the carboxy-terminal tail by Csk renders the enzyme less active (2). |
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| Lyn is a member of the Src family that is predominantly expressed in hematopoietic cells (3). Lyn participates in signaling from multiple cell surface receptors such as the B cell antigen receptor (BCR) and CD40 (4). Lck is essential for T-lymphocyte activation and differentiation |
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| (5,6). Phosphorylation of the carboxy-terminal Tyr505 downregulates Lck activity, while phosphorylation at Tyr394 leads to an increase in Lck activity (7). |
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| Catalog # | S6500-06E |
| Applications | Suitable for use in Western Blot. Other applications not tested. |
| Recommended Dilution | Western Blotting: 1:1000. Incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking overnight. Optimal dilutions to be determined by the researcher. |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot. Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Host | Rabbit |
| Source | Human |
| Concentration | N/a |
| (10 Western mini-blots) |
| Form | Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol. |
| Purity | Purified by Protein A and immunoaffinity chromatography. |
| Immunogen | Synthetic phospho-peptide corresponding to residues surrounding Tyr416 of human Src. |
| Specificity | Detects endogenous levels of Src only when phosphorylated at tyrosine 416. The antibody may crossreact with other Src family members (Lyn, Fyn, Lck, Yes and Hck) when phosphorylated at equivalent sites. It does not crossreact with Src phosphorylated at tyrosine 527. It may crossreact with phosphorylated RTKs. Species Crossreactivity: hamster, human, chicken, monkey, mouse, rat, bovine, xenopus, zebra fish. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
