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You are here:Home » Antibodies » Abs to Protein Kinase Substrates » Anti -Src, phosphorylated (Tyr215) (Proto-oncogene Tyrosine-protein Kinase Src, p60-Src, c-Src, Proto-oncogene c-Src, pp60c-src, SRC1)

Anti -Src, phosphorylated (Tyr215) (Proto-oncogene Tyrosine-protein Kinase Src, p60-Src,
c-Src, Proto-oncogene c-Src, pp60c-src, SRC1)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
c-Src was the first proto-oncogenic non-receptor tyrosine kinase characterized in human. The Src family is composed of nine members in vertebrates, including c-Src, Yes, Fgr, Yrk, Fyn, Lyn, Hck, Lck, and Blk. Src-family kinases transduce signals that are involved in the control of a variety of cellular processes, including proliferation, differentiation, motility, and adhesion. Src-family kinases contain an amino terminal cell membrane anchor followed by an SH3 domain and an SH2 domain. The activity of c-Src is regulated by tyrosine phosphorylation at multiple sites. Tyrosine 418 is autophosphorylated following c-Src activation. Tyrosine 215 in the SH-2 domain of c-Src is phosphorylated following growth factor receptor activation. Both Tyr215 and Tyr418 phosphorylation increases tyrosine kinase activity, while phosphorylation of Tyr529 downregulates c-Src kinase activity. Thus, tyrosine phosphorylation of c-Src is critical for regulating its kinase activity.
Catalog #S6500-10H
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000
Optimal dilutions to be determined by the researcher.
Positive ControlPervanadate treated A431 cells
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in PBS, 1mg/ml BSA, 0.05% sodium azide, 50% gylcerol.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to aa residues around tyrosine 215 of human c-Src (KLH).
SpecificityRecognizes human c-Src, phosphorylated at Tyr215. Species Crossreactivity: mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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