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You are here:Home » Antibodies » Abs to Protein Kinase Substrates » Anti -Src family, phosphorylated (Tyr416)

Anti -Src family, phosphorylated (Tyr416)

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Supernatant B
Src (also known as pp60src) is a non receptor Tyrosine Kinase involved in signal transduction in many biological systems and implicated in the development of human tumors. There are two critical phosphorylation sites of tyrosine on Src, tyrosine 418 and tyrosine 529 (referring to human Src sequence). The tyrosine 418 is located in the catalytic domain and is one of the autophosphorylation sites. Full catalytic activity of Src requires phosphorylation of tyrosine 418. This region of Src is also highly conserved in all of the related Src-family kinases, and thus prior immunoprecipitation may be required to identify which Src family member is being activated.
Catalog #S6504-25B
ApplicationsSuitable for use in Western Blot.
Other applications not tested.
Recommended DilutionWestern Blot: 1: 1000
Optimal dilutions to be determined by the researcher.
Positive ControlRIPA cell lysate
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG
Clone No10F664
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in PBS, 0.05% sodium azide.
PuritySupernatant
ImmunogenSynthetic peptide containing the sequence ...NE[pY]TA…, where pY corresponds to phosphotyrosine at position 416 of c-Src.
SpecificityRecognizes vertebrates phospho-Rsk3 (Thr353/Thr356). Sequence homology: mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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